FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354699
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC22740.16786016672338283No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC22570.1666052754154244No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA19770.14593647740199114No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA19750.14578884313046664No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT18880.13936675231914988No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT18390.13574971266679903No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG17010.12556294793160697No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT16940.1250462279812711No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC15760.11633580596132424No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA15590.11508091465336581No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC15180.11205441208711309No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA14880.10983989801424522No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT14260.10526323559698501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT46950.048.178882
GGGCGAC15650.048.1788757
CGATCTG22500.048.0110745
CCGATCT47500.047.7736474
CTTCCGA48150.047.1304661
GGCGACC18000.041.8888558
GCGACCC18650.040.817669
TCCGATC66550.034.752043
CAGGGGC4500.032.2221954
CGGGCCG4400.031.30795145
CGATCTC20800.029.9759395
TAGAGTG7600.029.5723465
CCTATAA16050.029.362431
GGGGCGA31050.028.9532816
CTATAAG16900.027.0266062
GTACAGG3555.456968E-1226.5502571
TATAAGC17500.026.5142653
AAATTAG7450.026.2761151
TAGGACA8300.026.20484
TAATACT4500.025.7777564
Produced by FastQC (version 0.11.2)