FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980864
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16130.1644468550176171No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC16070.16383514941928748No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15530.15832979903432076No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15070.15364005611379355No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT12630.12876402844838836No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT12390.12631720605506983No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT12380.1262152551220149No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG11390.11612211274957587No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11320.1154084562181913No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11180.11398114315542215No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC11050.11265578102570796No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC10360.10562116664491712No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA10010.10205288398799427No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT9860.10052361999217016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTA1002.7340957E-643.499985145
CTTCCGA52950.034.232141
CGATCTG21200.034.19815
TTCCGAT54050.032.9972152
CCGATCT54550.032.827674
CCTACAT4500.028.999993
GGGCGAC10250.028.999997
CGGGCCG3950.027.531637145
TCCGATC67750.026.6457483
TAGACGT3607.2759576E-1226.1805484
TATACTG3607.2759576E-1226.1805485
GCGACCC11800.025.8050759
CTATAAG10750.025.6278972
CCTATAA10250.025.4647051
TGTAGGA7800.025.0961442
GGCGACC12000.024.7708268
TAGAGTG6200.024.5564445
GTCCTAA7800.024.1678891
CGATCTA12750.023.8823435
CCTGTAC3052.0345396E-823.7704853
Produced by FastQC (version 0.11.2)