FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615145
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC12070.1962139007876192No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC11430.18580984971023093No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10610.17247965926732722No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10250.16662738053629633No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8620.1401295629485731No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT7650.12436092303440652No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA7200.1170455746206179No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6760.10989278950491348No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT6400.10404051077388257No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT6310.10257744109112485No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC6170.10030155491794618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT15000.058.483144
TTCCGAT15100.057.61572
CTTCCGA15150.056.956261
CGATCTG7250.045.9998475
CGATCTA3650.039.7258955
TCCGATC23700.037.320553
TCGCGCG3250.035.692199
CGAGGGG1707.8091034E-834.120304145
GGGCGAC6300.032.222117
CAGGGGC1902.0744483E-730.5262134
TATAGGT4050.030.4319973
CCATACT5050.030.1484132
GGCGACC6800.029.852848
TCCCGGG1952.604629E-729.7434882
ACTCCCC5050.028.7127746
GTCGCGC4050.028.641888
GCGACCC7150.028.3915149
CTCCCCC4600.028.369477
AGGTCGC4600.028.369476
TATTAAT2104.981339E-727.6189542
Produced by FastQC (version 0.11.2)