FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725050
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10750.14826563685263086No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC10660.14702434314874835No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10550.14550720639955866No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA9470.13061168195296877No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8440.11640576511964691No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA7440.10261361285428591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAAC2103.783498E-1034.5237462
CTTCCGA50450.030.6179031
TTCCGAT50350.029.80632
GTCTAGG1952.5993904E-729.751741
CCGATCT50100.029.3761924
CGATCTG21300.028.2511225
AGGTGCC5700.027.9824037
CCTAGAC1306.4812903E-427.8845623
TAAGGTG6400.027.1874475
ATCTGGC3750.027.0666187
TGCGACC3005.857146E-1026.5832825
GCCTATA1100.00965695726.3708611
CATAAGG6650.026.1653633
CGATCTA9200.026.0053845
ATACCCG5100.025.5881865
TCCGATC58900.025.4795783
GTACTAA2051.2868915E-524.762881
CACGAGC3902.0008883E-1124.166622145
TACACAG3952.3646862E-1123.8607125
GTAGGCA3352.1082087E-923.8059233
Produced by FastQC (version 0.11.2)