FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236355
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16100.6811787353768696No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7040.2978570370840473No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.21281546825749403No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.19462249582196273No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG3260.13792811660426055No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACAG400.006045193454.3753
TAGGTGT604.93737E-448.3333325
ACTTAAT450.009630148.3333328
CATAGGT657.3251734E-444.6153833
CTATACT700.001055002641.428574
GTCTAGG700.001055002641.428571
TAGGGCT700.001055002641.428574
TACTGGG1601.0040822E-940.781252
TCTAGGG1252.7071837E-740.6000022
CTGTCCA1354.9499795E-737.592599
TCTGTCC1209.532987E-636.258
TCTAAGC800.002033475636.253
GATCTTT8650.035.2023126
CTAGGGC850.002737770834.117653
ATACCGG1102.430552E-432.9545486
AGTAATA1102.430552E-432.9545482
CAGTAAT900.003622670432.222221
CGATCTT14000.032.107145
ATAGGTG1153.1548526E-431.521744
CGATCTG21050.031.3420435
Produced by FastQC (version 0.11.2)