FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377287
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18400.4876923933239152No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.26982111761073135No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG6870.18208949685517922No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG6160.1632709316780064No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.16115052996790244No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5400.1431271154320186No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC4710.12483865068237178No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.12086289747592681No Hit
GTACAGGAATATTCACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATC4330.11476674255937788No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3870.10257443272627999No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG951.02767364E-438.1578948
CTCTAGG1209.548072E-636.251
GATCTTT13900.034.946046
ATCTAGC2353.45608E-1133.9361727
TCTGACC1751.0063559E-733.1428578
CGTCTAA1102.4332889E-432.9545441
CTAGACG2950.031.9491543
CGATCTT22700.031.9383245
ATCTAGG1153.1583948E-431.521741
GTAGGGA2351.2569217E-930.8510653
TTCTAAG950.004723603430.5263182
GCCTAGG1204.0533041E-430.2083341
TGTAGAC1204.0533041E-430.2083342
TTAATAC1453.4846955E-530.03
CCGATCT100650.029.3169384
CTTCCGA102200.029.2270051
TCTGTCC3001.8189894E-1129.0000028
CTGTAGG1255.147833E-429.01
ACTAGGG2502.4374458E-929.02
TCTAAGG1255.147833E-429.03
Produced by FastQC (version 0.11.2)