FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7c12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7c12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327408
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14250.43523676880222845No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.17409470752089137No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.1346943263451107No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.11331427454429946No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGA502.0082385E-458.01
TCTATGA700.001055599241.428572
TATAGTA750.001481927938.6666642
CTATATA800.00203461836.251
TCTAGAC1655.995753E-835.1515162
CTATAGT850.002739305434.1176451
AGGGATG1301.6513979E-533.4615365
TGTAAGG900.003624694732.222222
TGACTTT900.003624694732.222226
CCGATCT53150.030.9642524
CGATCTT14300.030.9265735
CTATACT950.00472270330.5263144
GAGCGTA950.00472270330.5263149
TAACTTA950.00472270330.5263144
CTTCCGA54850.030.1367381
TTCCGAT54400.030.1194842
TCCGATC54500.030.064223
TGGACCA1453.483302E-530.05
GATCTTT9250.029.7837836
TAGACGT3900.029.743594
Produced by FastQC (version 0.11.2)