FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791997
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15070.19027849852966616No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC14840.18737444712543103No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA13660.17247540079065954No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA13430.16957134938642443No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT11840.14949551576584255No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10990.1387631518806258No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC10180.1285358404135369No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG9730.12285400070959865No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA9490.11982368620083157No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT9150.11553074064674489No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9110.11502568822861703No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT8720.11010142715187053No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8340.10530342917965597No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC8290.10467211365699618No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA8210.10366200882074048No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8140.10277816708901676No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA7990.10088422052103732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA24250.050.827931
CCGATCT25200.048.0456124
TTCCGAT25900.046.4671632
CGATCTG11700.043.995715
GGGCGAC12400.040.3427287
GTCTAGG1406.6040593E-736.2522741
TCCGATC35150.034.238963
CGATCTA6000.033.833325
GGCGACC14900.033.087238
GGGTACC2850.033.070168
TAGACGT2008.94579E-932.6249854
CCTATAA10400.032.0693171
GCGACCC15850.031.5615029
TAGAGTG5550.031.3513375
GTCGCGC5850.029.7435788
TCGCGCG5700.029.2543729
CTGGGCG1504.3975786E-528.9999899
CTATAAG11500.028.9999872
CGATCTT8350.028.6526815
CAGGGGC2802.6193447E-1028.482134
Produced by FastQC (version 0.11.2)