FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239456
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14650.611803421087799No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.24138046238139782No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG4330.18082654015769076No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.1319657891220099No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2940.12277829747427502Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC2820.11776693839369236No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2760.11526125885340105No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG2710.11317319256982494No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA451.19202035E-464.444357
TACACCA451.19202035E-464.444355
ATAGACT350.003563213462.1427653
ATAATTC400.006045324754.374923
ACGTACA400.006045324754.374922
GTCCTAA604.932451E-448.3433571
GCCTAGA604.932451E-448.3433571
GTATACG450.00962236648.3433571
TACTACA450.0096303148.3332632
ATACGGC604.937535E-448.3332633
TATACGG604.937535E-448.3332632
TCTAGGG1453.8198777E-1044.9999352
CGTACAC907.4654425E-540.277723
GTACTGG1502.5806003E-838.6746861
GGTGGCA2201.6370905E-1136.249959
CTAAGAT1001.3883035E-436.2499474
GGGCCCT900.00362279132.2221766
TAAGCAT900.00362279132.2221764
TCTAGAC3450.031.5216922
CTAGACG3250.031.2307263