Basic Statistics
Measure | Value |
---|---|
Filename | H55FTAFXY_n01_p7c10.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 239456 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1465 | 0.611803421087799 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 578 | 0.24138046238139782 | No Hit |
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG | 433 | 0.18082654015769076 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 316 | 0.1319657891220099 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 294 | 0.12277829747427502 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC | 282 | 0.11776693839369236 | No Hit |
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC | 276 | 0.11526125885340105 | No Hit |
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG | 271 | 0.11317319256982494 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGCCCTA | 45 | 1.19202035E-4 | 64.44435 | 7 |
TACACCA | 45 | 1.19202035E-4 | 64.44435 | 5 |
ATAGACT | 35 | 0.0035632134 | 62.142765 | 3 |
ATAATTC | 40 | 0.0060453247 | 54.37492 | 3 |
ACGTACA | 40 | 0.0060453247 | 54.37492 | 2 |
GTCCTAA | 60 | 4.932451E-4 | 48.343357 | 1 |
GCCTAGA | 60 | 4.932451E-4 | 48.343357 | 1 |
GTATACG | 45 | 0.009622366 | 48.343357 | 1 |
TACTACA | 45 | 0.00963031 | 48.333263 | 2 |
ATACGGC | 60 | 4.937535E-4 | 48.333263 | 3 |
TATACGG | 60 | 4.937535E-4 | 48.333263 | 2 |
TCTAGGG | 145 | 3.8198777E-10 | 44.999935 | 2 |
CGTACAC | 90 | 7.4654425E-5 | 40.27772 | 3 |
GTACTGG | 150 | 2.5806003E-8 | 38.674686 | 1 |
GGTGGCA | 220 | 1.6370905E-11 | 36.24995 | 9 |
CTAAGAT | 100 | 1.3883035E-4 | 36.249947 | 4 |
GGGCCCT | 90 | 0.003622791 | 32.222176 | 6 |
TAAGCAT | 90 | 0.003622791 | 32.222176 | 4 |
TCTAGAC | 345 | 0.0 | 31.521692 | 2 |
CTAGACG | 325 | 0.0 | 31.230726 | 3 |