FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152744
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12800.838003456764259No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.3646624417325722No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.2121196249934531No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.17611166396061384No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG1860.1217723773110564No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.10016760069135285No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTT400.0060411154.3750044
CTCATAA400.0060411154.3750041
ACCTATA752.5403548E-548.3333361
TCATAAG604.931841E-448.3333322
CTAGGGA1402.6921043E-1046.6071433
ATACCCG803.719712E-545.3125045
ACTAGGG700.001053828241.428572
TCTAGGG1209.510886E-636.252
GATCTTT6200.035.0806476
CGATCTT9050.033.6464085
TATAGGT1301.6456544E-533.461543
TATACCC1301.6456544E-533.461544
ATAGGTC1352.1304118E-532.222224
ATCTGAG900.003618686232.222227
TACCCGG1153.1496608E-431.521746
ATCTTTT6300.029.9206357
TAGACGT3750.029.0000024
CTAGACG2003.2239222E-729.03
CTATACC1255.133664E-429.03
CCTATAC1255.133664E-429.02
Produced by FastQC (version 0.11.2)