FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970534
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC18220.18773170234118536No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC16370.1686700311374975No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15480.15949982174761523No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA14920.15372980235622863No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT13090.13487420327366173No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA10900.11230930601091771No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10900.11230930601091771No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT9790.10087230328870499No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA23050.055.6781461
CCGATCT24150.053.4368134
TTCCGAT24850.051.63982
CGATCTG10400.050.889395
TCCGATC36300.036.1501123
TCCCCCC5900.033.1779448
GGGCGAC8400.032.7975967
CGAGGGG2601.10958354E-1030.673056145
CTCCCCC6650.030.5262937
TCGCGCG5050.030.1484939
GTACAGG2901.2732926E-1130.0030691
CGATCTA6350.029.6850175
GCGACCC10700.029.1354949
TAGCGTG1504.398595E-528.999985
ATACTCC7350.028.6054214
GGGTACC2802.6375346E-1028.482128
GTCGCGC5450.027.9357598
CCTACAC7800.027.8845983
TATACGT1855.8159367E-627.4324114
TCTTCCG15750.027.1587095
Produced by FastQC (version 0.11.2)