FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971606
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC20300.20893242734194725No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC17470.17980539436767579No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA16570.17054238034758945No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16170.16642548522755107No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT14340.14759069005337555No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11670.12011041512711942No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11300.11630228714108394No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT10920.11239123677704747No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA10430.10734804025500047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA30950.048.0234571
CCGATCT32250.046.0852554
TTCCGAT32350.045.94282
CGATCTG15700.038.7897955
GGGCGAC6700.037.8731237
CGATCTA7850.034.1719635
TCCGATC44600.033.4865343
GGCGACC8400.030.2083238
GCGACCC9150.029.316939
CGATCTT12200.028.5245785
GGGTACC2705.5624696E-926.8518418
TAGGACA4400.026.3636264
TAGAGTG7200.025.1736035
TCTTCCG21950.025.1024975
TATACAG2901.1905286E-824.999995
TGCCTAC4400.024.7171731
GTACTAT1801.5197152E-424.1679021
GTATAAA3952.3646862E-1123.8619791
CCTACAC7050.023.6524733
CTACACC8000.023.5624924
Produced by FastQC (version 0.11.2)