FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627942
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15170.24158282134337247No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.1334518156135439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT16850.031.8421616
CGATCTT25850.030.57295
CCGATCT112100.030.3345744
TTCCGAT113850.030.123042
CTTCCGA116350.029.8544451
TCCGATC116350.029.475793
CGATCTG43900.029.3985655
ATCTGTG5450.027.935667
GATCTGG13300.026.7125366
ACAGTCC1907.150129E-626.710418
ATACCCG2507.815288E-826.1019655
TGTACCG1409.986052E-425.8948065
ATCTTTT19150.025.744017
CTAGGGA5500.025.047343
ATCTCGC6200.023.3869957
TAGACGT9000.023.362874
GTCTACA4900.022.1955472
GTCCTAA3955.857146E-1022.0304831
TCTAGAC5600.022.0105842
TAGGGAG14500.022.0016564
Produced by FastQC (version 0.11.2)