FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781161
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC18000.23042625015841806No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC17530.2244095647376149No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15610.19983076472071698No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA15250.19522223971754862No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT13510.17294770220223488No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11270.14427243551585395No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11050.14145611468058442No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT10090.12916671467213545No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA9500.1216138542502762No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT9140.11700532924710783No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9120.11674930008026514No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT9120.11674930008026514No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG9020.11546915424605171No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC9000.11521312507920903No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT8610.11022055632577664No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG8520.10906842507498453No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT8500.10881239590814186No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA8390.10740423549050707No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT8310.10638011882313633No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA8270.10586806048945095No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC7940.10164357923654663No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG7870.10074747715259721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT22800.048.6542364
CTTCCGA23200.048.4466131
TTCCGAT23750.047.013352
CGATCTG11050.046.5865025
GGGCGAC6550.036.528917
GGGTACC3000.036.2498558
CGATCTA5100.032.698045
TCCGATC34800.032.2936063
TCCCCCC6300.028.7697288
TAGAGTG6050.028.7620565
GGTACCT3850.028.2466439
GGCGACC8500.028.1469468
ATATAGG1306.474544E-427.8898621
GCGACCC9350.027.138939
TCCTACA6700.027.0538642
CGATCTC10300.026.749185
CCATACT7600.026.7121322
TATTAGG1907.149474E-626.7121322
CTCCCCC6800.026.6560127
ACCATAC7350.026.6376651
Produced by FastQC (version 0.11.2)