FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353145
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC27690.20463438877577791No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC25940.19170155452667675No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA25100.1854937940871082No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA23770.1756648400577913No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT21940.1621407905287312No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT17300.1278503042911144No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA16950.12526373744129415No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT15390.11373503948209543No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT15150.11196139364221867No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT14130.10442339882274258No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT14040.10375828163278882No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA13990.10338877208281448No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA13850.1023541453428864No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG13820.10213243961290179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA43600.048.5568241
CCGATCT43950.048.168364
TTCCGAT45550.046.794722
GGGCGAC9800.043.647957
CGATCTG20600.037.3058135
GCGACCC12300.034.186989
TCCGATC63800.033.6363563
GGCGACC12700.033.1102268
CGATCTA12600.032.222215
CAGGGGC4400.031.306814
TCGCGCG7950.031.006289
CGAGGGG2951.4551915E-1129.491518145
TAAGGTG13500.027.925925
GTCGCGC8650.027.6589558
AGGTGCC12850.026.5175047
CTAGGGG3357.4578566E-1125.9701423
GGTCGCG9250.025.8648577
CATAAGG16050.025.2959463
CGATCTT17000.025.1617585
CGATCTC21450.025.011655
Produced by FastQC (version 0.11.2)