Basic Statistics
Measure | Value |
---|---|
Filename | H55FTAFXY_n01_p7b2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 271096 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2467 | 0.9100097382477057 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 891 | 0.32866586006433146 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 632 | 0.23312774810399267 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 606 | 0.22353704960604362 | No Hit |
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 330 | 0.12172809632012276 | No Hit |
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG | 310 | 0.11435063593708503 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 301 | 0.11103077876471804 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 283 | 0.10439106441998407 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACTAAGT | 10 | 0.007088059 | 144.99982 | 8 |
GTACATA | 35 | 0.0035611135 | 62.154243 | 1 |
CTTATAT | 40 | 0.0060461783 | 54.37493 | 2 |
CTAACGT | 80 | 3.7274356E-5 | 45.312443 | 3 |
GTTTAAT | 80 | 0.0020321729 | 36.25664 | 1 |
AGGGATG | 145 | 8.66743E-7 | 34.999954 | 5 |
TACGCTG | 110 | 2.4315083E-4 | 32.954502 | 5 |
CTACCCT | 135 | 2.1364993E-5 | 32.222183 | 4 |
CCTAACG | 120 | 4.0503536E-4 | 30.208292 | 2 |
TACACTG | 120 | 4.0503536E-4 | 30.208292 | 5 |
TCTAGGG | 175 | 3.7613136E-6 | 28.999964 | 2 |
CTATCAC | 100 | 0.006066792 | 28.999962 | 3 |
TGTAGAC | 125 | 5.1440945E-4 | 28.999962 | 2 |
TTCTAGA | 105 | 0.007691821 | 27.62411 | 1 |
ACTGTGT | 105 | 0.0076987515 | 27.619013 | 8 |
TCTATCA | 105 | 0.0076987515 | 27.619013 | 2 |
AGGTGTT | 290 | 3.929017E-10 | 27.499966 | 6 |
GATGGCA | 300 | 5.820766E-10 | 26.5833 | 8 |
TGTAACA | 165 | 8.396436E-5 | 26.363604 | 2 |
CAGATTC | 110 | 0.009659451 | 26.363602 | 4 |