FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271096
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24670.9100097382477057No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8910.32866586006433146No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.23312774810399267No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.22353704960604362No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.12172809632012276No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG3100.11435063593708503No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA3010.11103077876471804Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.10439106441998407No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGT100.007088059144.999828
GTACATA350.003561113562.1542431
CTTATAT400.006046178354.374932
CTAACGT803.7274356E-545.3124433
GTTTAAT800.002032172936.256641
AGGGATG1458.66743E-734.9999545
TACGCTG1102.4315083E-432.9545025
CTACCCT1352.1364993E-532.2221834
CCTAACG1204.0503536E-430.2082922
TACACTG1204.0503536E-430.2082925
TCTAGGG1753.7613136E-628.9999642
CTATCAC1000.00606679228.9999623
TGTAGAC1255.1440945E-428.9999622
TTCTAGA1050.00769182127.624111
ACTGTGT1050.007698751527.6190138
TCTATCA1050.007698751527.6190132
AGGTGTT2903.929017E-1027.4999666
GATGGCA3005.820766E-1026.58338
TGTAACA1658.396436E-526.3636042
CAGATTC1100.00965945126.3636024