FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398747
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27710.6949268583838876No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.2879018525531227No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.17605148126506281No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.15072213709444837No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG5520.13843364338791264No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG4540.11385665597484118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGC2250.038.66667
ACCGTAT750.00148232838.66668
TCTAGGC1001.3899637E-436.249948
TGTAGAC1301.6523165E-533.4614832
CGATCTT24100.033.392065
GATCTTT15100.033.129086
GTCTAGG1551.4592388E-632.750091
TCTAGAC4250.032.411712
TCTAGGG2558.731149E-1131.2744562
CTAGACG4450.030.9550043
GGCTACA950.004718168630.533921
ATCTGAG2859.094947E-1230.5262627
TAGACGT7400.030.371574
TAGAGTG1204.053755E-430.2082825
ATCTAGA3650.029.8019431
TTCTGGG2451.9645086E-929.5917842
TACAGGG2951.4551915E-1129.4914742
TACTAAT1255.148402E-428.9999522
CTTGCTA1000.006070188728.999959
TTAGTAC1000.006070188728.999953
Produced by FastQC (version 0.11.2)