FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7b12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7b12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627752
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC26330.16175682782143716No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC26000.1597294919619205No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA23040.1415449036462557No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA21600.1326983471683647No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT21150.1299337982690238No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT19100.11733974217202621No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG18270.11224068531324183No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT18170.11162634111338827No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA17740.10898466105401805No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA17400.10689589077451601No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC16760.10296408789545336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC13400.037.3320167
CGATCTG36700.036.1511575
CTTCCGA89700.035.9758831
CCGATCT91500.035.101034
TTCCGAT91850.034.9683462
GCGACCC15700.030.9394329
GGCGACC16500.030.7575158
TCCGATC113150.028.5770573
CGATCTA21250.026.2705385
CCTATAA19800.026.0038151
CTAACGT9600.025.6770343
TACGAGT16800.025.4612624
CGAGTCG16800.025.0297156
CTATAAG20050.024.9508442
GGGTACC4201.8189894E-1224.1666226
GTCTACG18500.023.9112361
GTCTAGT3402.5011104E-923.4616031
CCTACTC11800.023.3474123
ACGAGTC18150.023.1685
TATACTG5350.023.0373385
Produced by FastQC (version 0.11.2)