FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7b11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7b11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408830
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17600.43049678350414594No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6150.1504292737812783No Hit
GTGCAGGAATCTTGACCTGCTGTCCATCGGCTGCGCCTTTCGGCCTCACC5970.1460264657681677No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.13771005063229216No Hit
TTCCTGCACCGTGTACATGGAGTGATGGAGGGACGCATTACGCTATTCCA4900.119854218134677No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4540.11104860210845582No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.1078687963212093No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG4310.10542279186948121No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAC1303.6841993E-739.0479471
CGATCTT17200.036.671455
ATATAGT850.002740120734.1175884
CCGATCT74550.033.8430024
GATCTTT7850.033.2483526
TTCCGAT76550.032.9587942
TCCTAGG1102.4336667E-432.954492
TCTGTGT1102.4336667E-432.954498
CATAATC1102.4336667E-432.954493
CTTCCGA77450.032.583771
TCCGATC79050.032.1915973
TACACTG1153.158886E-431.5216855
ATTACAT950.00472410130.5262643
ATACGTA950.00472410130.5262644
ATGGGCC1204.053932E-430.2082824
TCTGTGC2401.5752448E-930.2082828
ATCTCAT2202.2573658E-829.6590427
ATCTTTT7150.029.4055447
TCTAGGG1753.7674872E-628.999952
TCTAACG1000.00607036428.999952
Produced by FastQC (version 0.11.2)