FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200584
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG38750.32275959033270474No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC28780.23971667122000626No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC27150.22613994522665634No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG26000.21656127351355672No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT20100.1674185229854804No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA19050.15867277924743292No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA18550.15450813937217223No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA17810.14834447235678636No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15990.1331851832108374No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT15580.1297701785131236No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA15040.12527236744784204No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC15020.1251057818528316No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG14460.12044138519253962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT133500.050.234052
CCGATCT133700.050.213144
CGATCTT32500.049.0768935
CTTCCGA137350.048.935611
TCCGATC142900.047.0311133
GATCTTT19000.041.2105036
CGATCTA27400.041.012755
CGATCTG59200.039.556575
ATCTAGC5900.039.3220147
ATCTGGC7450.036.9798437
CGATCTC46500.035.8601955
ATCTTAC3450.035.7246177
GATCTGG20800.034.8557476
CTTATAA5100.034.117626145
GATCTAC13000.033.461526
ATCTAAC3700.031.3513347
TACACAA7650.031.27449145
GTCTAGG1902.075667E-730.5288411
ATCTGAC6900.030.4709997
ATTACAC4850.029.896893
Produced by FastQC (version 0.11.2)