FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320331
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20930.6533866531806163No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8420.2628531113129856No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG6700.20915865151983418No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.16295644193037828No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4620.14422581642113938No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC4480.13985533713565032No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.1261195450955418No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG3820.11925164907548753No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3580.11175939887179198No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3320.10364279448445515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT1600.054.3749964
CTAGACG1307.3232513E-944.6153833
CTGTATG657.3290884E-444.6153839
GTAGACG657.3290884E-444.6153833
CTTCCGA42950.043.719441
CCGATCT43050.042.9442524
GATCTCG3900.042.7564136
TTCCGAT43750.042.7542882
TCCGATC46100.040.5748333
CGATCTG16900.039.038465
CGATCTT13700.036.51465
TCTAGAC1604.5729394E-836.2499962
CGATCTC13350.033.670415
GATCTAG2404.3655746E-1133.2291686
CGATCTA8150.032.0245365
TCAGGGG1601.8718529E-631.7187483
TGTAGAC1402.740899E-531.0714282
ATATGAC950.004722550530.5263183
GATCTTT10050.029.5771166
ATCATAC1000.00606837729.03