FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395431
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC30940.22172361084138162No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC29600.21212084295103092No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA28240.20237475016679435No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA27030.19370359408670154No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT23930.1714882355343976No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA19140.13716192344874092No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT18700.13400877578325263No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT18590.13322048886688054No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT17930.1284907673686481No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT17880.1281324551339335No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC17880.1281324551339335No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG17340.12426268299901608No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT16910.12118119778047069No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC16210.11616482649446658No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA15750.11286835393509245No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA15720.11265336659426371No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG15500.11107679276151955No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT15480.11093346786763374No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA14910.10684870839188752No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA14860.10649039615717294No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA14180.10161734976505467No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT14120.10118737508339717No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG14090.10097238774256843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA54000.045.1127171
CCGATCT54950.044.7270134
TTCCGAT54250.044.6359332
CGATCTG23000.038.1412965
CGATCTC20450.034.034225
GGGCGAC14450.033.615917
TCCGATC76400.032.4541783
CCATACT11150.031.8609772
TCGCGCG10100.031.584159
CATACTC12100.029.958673
GTCGCGC10750.029.6744128
TATAGGT11000.028.9999943
ATACCCG14350.028.7979035
GGCGACC16850.028.3976218
CCTACAC13600.028.2536683
TCCTACA12650.028.0829982
CGATCTA13750.027.9454465
TAGTACA2603.7234713E-927.8846074
ATACTCC12600.027.619044
ACCATAC12100.027.5629651
Produced by FastQC (version 0.11.2)