FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181877
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20201.110640707731049No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.34308901070503695No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.2204786751485894No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.19793596771444438No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3590.19738614558190426No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC2920.160548062701716No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2390.13140748967708946No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG2270.12480962408660799No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG2010.11051424864056476No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA1940.10666549371278392No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1890.10391638305008329No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.10226691665246293No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACTC100.007086408144.998084
TTCTAGA554.3744876E-879.089861
TGTATTC350.00356200162.1420338
TGTAGAC606.7838355E-660.4158632
CTAAGCT450.00962706348.332693
TTAAGGT604.9346674E-448.332694
CACTAGG450.00962706348.332691
TAGTACA450.00962706348.332694
GGTGGCA1351.0182703E-842.962397
CTCAACT700.001054428841.428029
TCTAGAC2005.456968E-1239.874472
ATCTCGA1354.9421214E-737.592097
ACACACG1001.3870858E-436.249521
GTCTAGG800.002032376636.249521
ATAACAT1604.5549314E-836.249523
CTAGACG2401.8189894E-1236.2495163
GTGGCAG1655.9713784E-835.1510478
TTTGGGC850.002736296334.117193
TAGACGT4700.033.935724
ATCATAG1501.1272496E-633.8328868
Produced by FastQC (version 0.11.2)