FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165124
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17931.0858506334633367No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.38819311547685376No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.2016666262929677No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.17986482885589014No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.11022019815411449No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG350.00356123362.1428571
TTACACG502.00531E-458.04
GTCGTAC752.5412606E-548.3333361
TATACGC450.00962499848.3333328
GATTGTA750.001479797838.6666687
TCGTACT1001.3864243E-436.252
TCAAGAC1001.3864243E-436.253
CGATCTT10800.034.236115
TCTAGGG850.002735390834.117652
GATCTTT7450.032.1140946
TTGAACA1402.732924E-531.0714284
CTAGGGA2351.2460077E-930.8510653
TCCTGGG1753.7495647E-629.02
CCTACGG1000.00605997729.01
TATAGAA1306.459874E-427.8846152
CCGATCT42300.027.2517744
ATCTTTT8700.026.6666667
CGATCTG14150.026.643115
TTCCGAT42700.026.487122
CTTCCGA43250.026.4855481
Produced by FastQC (version 0.11.2)