FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756561
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34320.4536316304964173No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.2199426087255357No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.12807956000904092No Hit
GTGTTAAGAAGAGGAATTGAACCTCTGACTGTAAAGTTTTAAGTTTTATG9070.1198845830012385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGC1550.051.451576
TCTAGGC1700.046.9117248
CTAGGCC1002.7332717E-643.499969
GATCTTT25200.033.948386
TTATACT4100.033.597534
CGATCTT42250.031.0591435
TCTAGGG4600.029.9456252
TTTATAC5000.028.9999753
TATACTG5050.028.7128435
ACTGCTC3600.028.1944188
TCTAGAC6400.027.1874752
GCTAACA1606.826018E-527.1874753
GTAGTAT2156.11426E-726.9802841
TACTGCT3503.6379788E-1226.9285497
GCCTTAG1358.074689E-426.8553771
CTCTAGG1358.074689E-426.8553771
ATACGTC1358.080927E-426.8518285
CCGATCT198200.026.1541124
ATCTTTT28900.026.0899437
CTTCCGA204750.025.6395071
Produced by FastQC (version 0.11.2)