FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7a11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7a11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105382
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9700.9204607997570743No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.3558482473287658No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.18788787458958836No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.14613501356967984No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACTT203.8563577E-4108.752
TTGACCC259.3632843E-487.04
TCTAGAC353.4229924E-582.857142
ATAGTAT350.003557655562.1428578
TAGACGT606.7635574E-660.4166684
TATACCG400.00603592954.3755
CTGCAGG553.2057785E-452.7272761
GGTGGCA701.6855598E-551.7857139
CTAGACG450.00961539948.3333323
ATACCGT450.00961539948.3333326
CTAGGGA1105.219552E-639.5454563
CAATTAT800.002028369836.251
ATTATAC800.002028369836.253
ACTGAGA950.004708324630.5263188
CTATTAA950.004708324630.5263181
TCTGCAG1000.00605015229.08
TCCTAAG1255.122986E-429.02
TCTGTGC1255.122986E-429.08
CTAATAC1000.00605015229.03
ATCTGTG1555.4545228E-528.0645147
Produced by FastQC (version 0.11.2)