FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p7a10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7a10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586986
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC30610.19288134866974252No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC28860.1818541562433443No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA26260.1654708989241241No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA24390.15368755615991572No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT23310.14688220311962424No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA19200.12098405404962614No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC18350.11562798915680415No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT17840.11241435022111097No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG17130.10794046072240082No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT17040.1073733479690432No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT16760.1056089971808195No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA16280.10258439582957884No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG16060.10119812021026021No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT43200.052.52894
CTTCCGA43850.051.7551421
TTCCGAT44600.050.7174572
CGATCTG22400.045.6361275
GGGCGAC22150.045.4965827
GGCGACC24250.041.5566758
GCGACCC26200.039.017159
GGGTACC6500.035.6922878
TCCGATC66450.034.258823
TCGCGCG10700.032.5233429
GTCGCGC11150.032.511198
CAGGGGC5450.031.9265864
GGTACCT7500.030.9333139
GGGGCGA34650.029.5021446
GTTCGAA29050.029.449205145
CTCCCCC12700.028.5432877
TAGGACA9150.027.7322224
GGTCGCG12450.027.3694617
TATACAG5050.027.277215
TCCCCCC13600.027.1874838
Produced by FastQC (version 0.11.2)