FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6h9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6h9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3266775
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC65020.1990342157020303No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC64070.1961261488777158No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA63540.1945037537020456No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA58420.1788308040804769No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT47950.1467808465535582No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT47920.14668901286436928No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT46220.14148510381033283No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC45970.1407198230670922No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT44490.13618936106710747No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT40770.12480198360768648No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG39810.12186330555364236No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT38930.11916951733743524No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT38830.11886340504013897No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA37610.11512883501312457No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC36510.11176159974286567No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT36370.11133304252665091No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA35690.10925147890503632No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA33950.10392512493208132No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG33440.10236395221587039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT70100.050.0574
CTTCCGA72850.048.1733061
TTCCGAT74450.047.2296262
CGATCTG39150.040.9258735
GGGCGAC19550.032.2633867
TCGCGCG22250.030.6291779
TCCGATC117000.030.2392793
TAGAGTG22800.030.2082965
GTCGCGC22700.030.0219888
CCTATAA44050.029.2998031
TATAGGT23950.029.0605053
CGAGGGG7600.028.618826145
CTATAAG46500.028.3763072
TAGGACA15100.027.8476474
GGTCGCG24600.027.7032177
TATAAGC49300.026.911733
TCCCCCC22900.026.9104468
AGGTCGC26450.026.587876
CCATACT25150.026.5208422
CTCCCCC24750.025.4848187
Produced by FastQC (version 0.11.2)