FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6h8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6h8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131697
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG62260.5501472567303792No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT57270.5060541823473952No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG45640.40328815928645206No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT43650.38570394725796747No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC41790.3692684525981778No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG36410.3217292261091087No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC35190.3109489554182789No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT34450.30441010270416907No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGA34050.30087558772356915No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC33690.2976945242410292No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA33620.29707598411942415No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA33330.2945134607584892No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT32440.2866491649266544No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG31520.27851978047127457No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT31430.27772451460063957No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC30760.27180420200813465No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT28680.253424724109015No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA28410.2510389264971101No Hit
CTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTAATCCGCG28250.24962512050487012No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG28210.24927166900681014No Hit
CCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTA28100.24829967738714517No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC28000.24741604864199515No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG27060.23910993843758535No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT26800.23681250370019535No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG26680.2357521492060154No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCG25800.22797621624869557No Hit
TTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGC24640.21772612280495574No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT24520.21666576831077577No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT24490.21640067968723078No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAA23890.2110989072163309No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC23890.2110989072163309No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT23530.20791784373379094No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT23160.20464841737673603No Hit
GTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTG22690.2004953622745311No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC22580.1995233706548661No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT22460.19846301616068615No Hit
ATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAA22410.19802120178811114No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA22340.19740266166650614No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA22040.19475177543105618No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA21070.18618057660310136No Hit
CTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGC21010.18565039935601138No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT20790.1837064161166814No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAAT20220.1786697322693265No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT19870.17557703166130156No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC19860.17548866878678657No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGC19840.17531194303775657No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA19830.17522358016324158No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAA19400.17142397655909664No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACT19260.17018689631588668No Hit
CCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGAC19200.1696567190687967No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG19190.16956835619428168No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT18990.16780109870398172No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA18840.16647565558625674No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT18790.16603384121368175No Hit
CATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCC18240.16117388311535685No Hit
ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTG18160.16046698011923688No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC18100.15993680287214687No Hit
ATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAA18080.15976007712311688No Hit
CCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGG17840.15763936813475693No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG17630.15578374776994197No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA17470.15436994177770197No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17370.153486313032552No Hit
CGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCA17110.15118887829516206No Hit
TGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCAC17070.15083542679710205No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG16810.14853799205971208No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGG16690.14747763756553212No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT16560.14632892019683716No Hit
GTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGA16520.14597546869877714No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTT16320.14420821120847718No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA16320.14420821120847718No Hit
GTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCT16280.1438547597104172No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCG16130.14252931659269222No Hit
GAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC15960.14102714772593725No Hit
GGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAAC15740.1390831644866073No Hit
TCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTCCTACCTAT15640.1381995357414573No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAG15550.13740426987082233No Hit
CGTCAGTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCG15350.13563701238052234No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC15200.1343115692627974No Hit
ACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCC15190.1342232063882824No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC15120.13360466626667739No Hit
GAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG15090.1333395776431324No Hit
CCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGT14900.13166068302734743No Hit
ATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCG14900.13166068302734743No Hit
GGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAATGATTAG14780.13060032853316744No Hit
ACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGT14740.13024687703510746No Hit
TCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAG14610.1290981596664125No Hit
GATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGCCAATCAGCTT14480.12794944229771749No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG14370.1269774506780525No Hit
CGCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGG14310.12644727343096251No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAA14180.12529855606226756No Hit
AACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG14090.12450329019163256No Hit
TCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGC14080.12441492731711758No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC13990.12361966144648258No Hit
GAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGA13980.1235312985719676No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC13930.1230894841993926No Hit
CCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGGTGCACCG13780.1217640410816676No Hit
GGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTA13600.12017350934039767No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAA13470.11902479197170267No Hit
ACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGA13420.11858297759912768No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAA13330.11778771172849269No Hit
CCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGC13260.11716917160688771No Hit
CCCAATGCCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCT13140.11610881711270774No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTC13130.11602045423819274No Hit
GGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGG13070.11549027699110274No Hit
ACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTTCCAC13060.11540191411658775No Hit
ACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAA13040.11522518836755775No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT13020.11504846261852775No Hit
CTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGAT13010.11496009974401274No Hit
CATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTG12980.11469501112046775No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12890.11389974524983278No Hit
GACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAA12880.11381138237531777No Hit
ACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGG12850.11354629375177278No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT12790.1130161165046828No Hit
CACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGA12730.11248593925759282No Hit
CACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTC12670.11195576201050281No Hit
GTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATT12640.11169067338695782No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12540.11080704464180784No Hit
GTCCTGGTCGGATGCGGAACGGAGCAATCCGGTCCGCCGATCGATTCGGG12540.11080704464180784No Hit
CCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACG12430.10983505302214285No Hit
ACTTGGAGCTCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGT12430.10983505302214285No Hit
CCTTACGGGTTTACTCACCCGTTGACTCGCACACATGTCAGACTCCTTGG12330.10895142427699286No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAG12300.10868633565344787No Hit
CTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGG12280.10850960990441787No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGT12160.1074492554102379No Hit
GTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGAAATTG12140.1072725296612079No Hit
AACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGC12080.1067423524141179No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTT12060.10656562666508793No Hit
GACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAA12060.10656562666508793No Hit
CTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGG12040.10638890091605792No Hit
CGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCG12000.10603544941799792No Hit
CCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAA11970.10577036079445293No Hit
GTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTT11960.10568199791993795No Hit
GTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGAT11950.10559363504542293No Hit
AGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAG11920.10532854642187794No Hit
GGTTTACTCACCCGTTGACTCGCACACATGTCAGACTCCTTGGTCCGTGT11770.10400310330415297No Hit
GCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGCTCCCCATTCGACCCGTC11600.102500934437398No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA11560.10214748293933801No Hit
GATTACGGTGGCGGCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGT11390.10064531407258304No Hit
TCCTTGCGCCTTACGGGTTTACTCACCCGTTGACTCGCACACATGTCAGA11360.10038022544903805No Hit
GTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCGCTCAG11320.10002677395097805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCATG1150.063.04342145
ACCTTTA702.3348366E-762.1428145
CTCTAGG502.0090505E-458.0076331
ATCTCTC502.0103673E-457.9999437
GAAGATA1154.0017767E-1156.73908145
CTTCCGA38050.056.0257721
TTCCGAT38350.055.769182
ATCTAAC1051.1095835E-955.238057
CCGATCT39000.054.0961044
ATCTGTG953.1399395E-853.4217
CGATCTA7600.052.4670525
CGATCTG15800.052.310085
CACATCA2650.051.981083145
GATCTAG2600.050.1922656
CTATAAC604.9453817E-448.333294
TATAACG450.00963949848.3332865
ACGATCC1700.046.91172145
CCTATTA1255.1968527E-946.39996145
AGGCTGG3750.046.39996145
GAACCAA951.9211238E-645.78943145
Produced by FastQC (version 0.11.2)