FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6h7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6h7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056882
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA33800.16432639305511934No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC32270.15688794981919235No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA28260.13739242212241637No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC27750.13491294104377402No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT23300.11327825320071837No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT22940.1115280312628532No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT22670.11021536480945432No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT21650.10525640265216965No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT21200.10306862522983817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA67850.047.6623421
CCGATCT68600.047.029844
CGATCTG29050.045.9207765
TTCCGAT70250.045.823132
TCCGATC96600.033.848313
CGATCTA16750.029.4328085
GGGCGAC10950.028.470297
TAAGGTG17500.027.7571165
AGGTGCC17050.026.363617
CCTATAA24950.026.1554571
GTCTACG19450.025.7228951
CGATCTC32150.025.4820885
CTATAAG26450.024.943872
CGAGTCG19100.024.6727496
GATCTGC29250.024.5384376
ACACATA14000.024.33926145
TCGCGCG13000.023.9807459
CCCACGC9700.023.9175145
TAGAGTG12800.023.789045
CACGAGC15700.023.550932145
Produced by FastQC (version 0.11.2)