FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6h5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6h5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220822
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12250.5547454510873011No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.3505085544012825No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5560.2517865067792158No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.16846147575875592No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.1403845631322966No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.13902600284391953No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG2560.11593047794150944No Hit
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT2510.11366621079421434Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTCTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT2290.10370343534611588Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGA953.1188392E-853.4330671
CTGTATG553.209935E-452.7391321
TACTACA1252.706147E-740.5999372
TGTAGAC1105.245729E-639.545392
TAGACGT4800.039.2707754
CGATCTG17900.038.882625
ATCTAGC951.02629994E-438.1578377
TCTAGAC2900.034.9999472
CCGATCT51000.034.686224
CGATCTT13800.034.673865
TTCCGAT51700.034.637282
CTCCTAC1051.8466102E-434.5315741
CTTCCGA52500.034.255321
ATAGACG850.002737403634.1175963
TCCGATC52900.033.8515553
GATCTTT10500.033.8332826
CTAGACG3250.033.4614873
CTCCGAC1102.4300595E-432.95449145
CTATAAG1102.4300595E-432.954492
TAAGCAA1102.4300595E-432.954495
Produced by FastQC (version 0.11.2)