FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6h3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6h3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019032
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA22360.21942392388070248No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC20470.20087691063676114No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC18370.18026911814349303No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA17490.17163347176536164No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT15080.14798357657070632No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT14430.1416049741323138No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT14420.14150684178710776No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA12900.12659072531578988No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12880.1263944606253778No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA12560.12325422557878457No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA12240.12011399053219132No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT11860.11638496141436186No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT11630.11412791747462296No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC11540.11324472636776862No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA10900.10696425627458216No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10720.10519787406087346No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT10560.10362775653757683No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC10220.10029125680057152No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT37000.038.6009984
CTTCCGA36600.038.4304921
TAGAGTG7150.036.5031625
TTCCGAT38750.036.4835362
GTGCTCA6900.035.7243089
CGATCTG17900.035.237115
GGGCGAC6800.035.1835027
GGCGACC7900.032.119968
GCGACCC8450.030.8872939
CTTATAA6900.028.369305145
CGATCTA7700.028.2464945
TTAGAGT9300.028.064264
ACACATA6100.027.335815145
TCCGATC52550.027.3165913
TCCCCCC7450.026.2749278
CCTATAA14700.025.1540641
GCGCCAT5200.025.0959266
TAAGGTG9250.025.080855
CCATACT8100.025.06152
AAATTAG9600.024.922871
Produced by FastQC (version 0.11.2)