FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6h11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6h11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155164
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC49210.22833529142097772No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC48310.2241592751178101No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA48300.22411287493666376No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA43920.2037895955945812No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT37040.17186627096592186No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT36090.16745825375702267No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT34910.1619830323817584No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC32840.15237819488447282No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT30800.14291255793062616No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG29450.1366485334758747No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT27850.12922450449246553No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA27170.12606929217451665No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT27160.12602289199337033No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC26630.12356368239261606No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT25880.12008366880664302No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT25590.11873806355340011No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA24490.11363404362730632No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA23870.11075723239623528No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG23840.11061803185279635No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG22660.10514281047753211No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG22620.10495720975294688No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG22170.10286920160136304No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA22160.10282280142021674No Hit
GCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACA21570.10008519073258462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT42950.046.082592
CCGATCT43100.045.249364
CTTCCGA45650.043.517841
AGACGCG11400.041.337658145
CGATCTG21250.040.599945
GGGCGAC13450.035.5761577
TCGCGCG16800.034.0922139
TATAGGT17250.032.7825623
GTCGCGC17500.032.7285238
GGTCGCG18550.030.8759657
ACACATA14250.030.017502145
TAGAGTG15600.029.2788055
TAGGACA9550.028.8481274
AGGTCGC20150.028.7840776
GTCCTAA13500.026.853061
TATACAG5750.026.4782225
CTTATAA13150.026.46384145
CGGGCCG4950.026.3636145
TTAGAGT17500.026.0999624
CCTACAC17900.025.921753
Produced by FastQC (version 0.11.2)