FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6h10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6h10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2778956
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA45300.16301085731476136No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC43720.15732526891393747No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA40250.1448385652741533No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC40160.14451470264372662No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC33750.12144848641000433No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT31310.11266821065176996No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT30930.11130079065663509No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT30760.1106890501324958No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT30240.10881784382336387No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT29580.1064428512002349No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT94250.040.230764
CTTCCGA95300.039.7128521
TTCCGAT98150.038.558322
CGATCTG46950.031.6560085
TCCGATC129200.029.796823
ACACATA20450.028.36185145
GGGCGAC15600.028.3493547
CGATCTA21950.028.0751655
TAGAGTG17950.027.8690745
ATAATAC8150.024.9079693
CCTATAA31600.024.5499291
TCGCGCG16550.024.5317159
TATAGGT16650.024.3843773
TAAGGTG22450.023.5746045
TTAGAGT22600.023.4181374
CTATAAG33700.022.1587492
GTATAAG9200.022.0660061
AGGTGCC23750.021.673687
AAATTAG22150.020.948831
GCGACCC19400.020.927839