FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6g9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6g9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451166
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA46310.18893049267165096No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC41370.16877681886906068No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC40630.1657578474897253No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA37890.15457949400407806No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT34880.14229962393407872No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT31090.12683759484261775No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT29020.11839263436258499No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA28100.11463931859368154No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT27990.11419055257783439No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA27140.11072281518265184No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT27100.1105596275405256No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT26520.10819340672969517No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA25490.10399132494494456No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG24740.10093155665507764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT67000.048.5857854
CTTCCGA68200.047.6294561
TTCCGAT68850.047.0696832
CGATCTG31400.041.791375
CGATCTA16650.032.6576355
TCCGATC104900.031.1010273
CCATACT21250.027.9764522
TCCCCCC18800.027.7659388
TAGAGTG17100.027.1344835
TCGCGCG14650.026.723539
ACCATAC21200.026.677231
CCTATAA37000.026.063451
CATACTC22850.026.0174873
CATACTA8950.025.921772
GGGCGAC16250.025.4307547
TTAGAGT19700.025.3933814
CTCCCCC20350.025.2948257
CGATCTC34650.025.1082085
ACTACCG8100.025.061715
TATATAC9550.025.0523383
Produced by FastQC (version 0.11.2)