FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6g8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6g8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594528
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA51220.1974154836640807No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC43860.16904808890094847No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC43790.16877829030945127No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA38820.14962259031315137No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT36950.1424151136545838No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT32370.12476257723948248No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT31260.12048434243145574No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT28750.11081013579348536No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA28570.11011636798677832No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA26980.10398808569419948No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG26880.1036026591349178No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT26790.10325577523156429No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT26500.10213803820964737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA50950.056.355831
TTCCGAT55150.052.189442
CCGATCT55550.051.9441534
CGATCTG28100.045.4092145
GGGCGAC12850.043.4435427
GGCGACC18350.031.6076038
TCCGATC94250.031.3076673
GCGACCC19350.029.9741389
TCCCCCC17600.028.8352038
CTCCCCC18400.028.3695417
CCCACGC6950.028.165445145
AGACCGC8300.027.951784145
AGGTGCC23600.027.6482837
TAGAGTG16950.027.374615
CGGGCCG5800.026.249979145
TAAGGTG25200.026.1805365
ACTCCCC20750.025.5060046
TCGCGCG14900.025.3019929
CCATACT21750.024.999982
CTAGGGG5000.024.649983
Produced by FastQC (version 0.11.2)