FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6g7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6g7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440899
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA28300.19640516094466026No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC26420.18335775095964393No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC24880.17266997895064123No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA21750.15094742934792793No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT21160.14685276344837495No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT18390.12762865405555837No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT17830.12374219150683011No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT16860.11701028316349724No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA16040.111319391574288No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT15960.11076418263875538No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC15590.10819634131191708No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT15500.1075717312594429No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT15180.10535089551731244No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA14620.10146443296858419No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG14440.10021521286363584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA35900.059.581371
TTCCGAT36150.058.7620622
CCGATCT37050.057.9216844
CGATCTG19700.051.1547855
TCCGATC55500.038.797273
ACACATA8600.033.720905145
CGATCTA9150.033.2786675
GGGCGAC8550.030.5262957
CCCACGC4850.029.896885145
CGATCTC18450.029.4715235
GTATAAG3700.029.3949281
GTCGCGC6950.029.2086148
TAGAGTG9700.029.1494645
AGGTGCC11800.028.8770967
GACCTCG6350.027.401556145
GTATATA5450.026.6082551
CCTATAA19500.026.0283321
TCGCGCG7850.025.8598549
GTACTAT2559.4827556E-825.590881
TAAGGTG14450.025.5882175
Produced by FastQC (version 0.11.2)