FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6g5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6g5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386132
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC14620.37862699802140204No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG13970.36179337635834374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11870.3074078294469249No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG11240.29109216537349925No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10330.2675250950452177No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC9930.2571659432525665No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA9740.25224534615105715No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA8810.2281603182331431No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8700.225311551490164No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.18413392311437538No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7080.18335698672992656No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA6690.17325681373209162No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA6490.16807723783576603No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT6240.161602767965359No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG5920.15331544653123802No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG5870.15202055255715663No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC5490.14217935835413797No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG5480.14192037955932169No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG5460.1414024219696891No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT5400.13984854920079143No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.1336330581252007No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT5120.13259714294593558No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC4890.12664063066516112No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT4880.12638165187034486No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC4720.12223799115328438No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA4700.1217200335636518No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4580.11861228802585644No Hit
ATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGT4440.11498658489842851No Hit
GATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAA4370.11317373333471455No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4270.11058394538655174No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT4270.11058394538655174No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG4160.10773517864357267No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC4100.10618130587467499No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT4050.10488641190059358No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA4000.10359151792651217No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT4000.10359151792651217No Hit
GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC3910.10126070877316566No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG3880.10048377238871681No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA553.2165798E-452.7270852
CTATGCG450.00963482548.3331649
ATCTGCG1950.044.6152237
TACACTG1002.7298283E-643.4998445
TGTAGAC855.3354917E-542.6469042
CTTAAGG1105.25603E-639.5453153
TACAGGA2200.039.5453152
AAGCACA1701.8226274E-938.3822141
ACACATA2151.2732926E-1137.097694145
CTTCCGA66200.035.921321
CCGATCT66450.035.677074
CGATCTG24500.035.510085
TTCCGAT67350.035.0926742
CTATACA1251.2631117E-534.7998774
TCTACAC2103.765308E-1034.523693
TATAATA1051.851653E-434.523692
TAGAGTG2950.034.406665
GCATAGG850.00273995934.1175231
TAGACGT5550.033.9638444
ATCTGAC2603.6379788E-1233.461427
Produced by FastQC (version 0.11.2)