FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6g3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6g3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096614
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA49860.23781201499179153No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC47690.2274619934809173No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC43090.20552185571593054No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA39950.1905453268937439No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT37080.17685658876645868No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT33660.16054457329770763No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT32490.15496414695313493No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA29300.1397491383726332No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT27620.1317362184932467No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT26930.12844519782849873No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA26710.1273958868919124No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC25510.12167237269235062No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA25480.12152928483736157No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT24830.11842904797926561No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT24480.11675968967106011No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG24250.11566268278281075No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA23810.1135640609096381No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT23770.11337327710298606No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC23610.11261014187637783No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA23090.11012995238990106No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG22100.10540805317526258No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG21960.10474030985198038No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC21520.10264168797880772No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG21430.1022124244138406No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA21190.10106772157392825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA43450.059.072091
TTCCGAT44500.057.6741262
CCGATCT45500.056.087884
CGATCTG25100.050.8366285
GGGCGAC13100.035.9732637
TCCGATC79600.032.1513633
TAGAGTG16750.032.029835
GGCGACC17350.028.8328368
TCTTCCG28400.028.5915365
TTAGAGT19400.028.4020464
CGATCTA10800.028.1944315
AAATTAG16800.028.052591
CTTATAA12700.027.401562145
CCCACGC5650.026.946888145
GCGACCC19750.026.4303659
AGGTGCC21100.025.4265297
CGGGCCG4600.025.21738145
TCGCGCG13350.024.981269
ACACATA13200.024.166655145
GTGCTCA19150.023.8511629
Produced by FastQC (version 0.11.2)