FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274186
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12720.46391865376058594No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7140.26040716885617793No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.20022904159949814No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.11853267489952077No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT3040.11087364052139788No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.10941477683032687No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA604.9387605E-448.3333321
TAAGGTT907.4682146E-540.277784
TTAGAGT1105.25032E-639.5454524
GTTTAAG951.0269396E-438.1578941
TTCTAGA1209.538549E-636.251
TCTAGGG1209.538549E-636.252
TACACCG1604.5689376E-836.255
CCTACTC900.003623673732.2222253
GTTCATA950.00472137630.5263181
CTTCCGA64500.029.3372081
CCGATCT64250.029.2256814
TAGAACC1504.3872147E-529.04
CATTATA1000.006066872729.02
AGCATAT1000.006066872729.06
CTATTAG1000.006066872729.01
AGTACAC1000.006066872729.03
TTCCGAT64950.028.7990762
CGATCTT15900.028.2704415
TCCGATC67050.028.005223
TTATATG1306.4708607E-427.8846154
Produced by FastQC (version 0.11.2)