FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6g1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6g1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331803
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA25950.19484863752371784No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC25570.19199536267751313No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC23690.17787916080681604No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA21380.16053425318909778No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT20080.1507730497678711No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT17440.1309502982047645No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT17010.12772159245774337No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA16180.12148943950419093No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT15430.11585797599194475No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT15270.11465659710933222No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA14530.10910021977724935No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT14430.10834935797561653No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT14400.10812409943512667No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC14070.10564625548973834No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA13560.10181686030141095No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT13510.10144142940059454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA39050.049.944241
CCGATCT38850.049.079784
TTCCGAT39600.048.1502382
CGATCTG19000.047.3157775
GGGCGAC8050.035.1242147
CGGGCCG3050.033.278683145
TCCGATC58700.032.4829563
CCTATAA17000.026.8686491
CGATCTA10550.026.8009415
TAGAGTG10100.025.8415785
GGCGACC10950.025.821918
GTCTACG15350.024.5611761
GCGACCC12150.024.4650149
ATACCCG10100.024.4059335
CGATCTC18900.024.1666625
CTAGGGG3001.708213E-824.1666623
CCCACGC3352.1118467E-923.805965145
CTATAAG20100.023.4452692
TAGTACA2502.2801687E-623.1999934
CAGGGGC4700.023.1382924
Produced by FastQC (version 0.11.2)