FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6g12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6g12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360402
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19000.5271890833014244No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.2608198622649153No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.20560374248755556No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.1356818219654719No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.12430563648370431No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.1054378166602849No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGA752.5431611E-548.3466531
TGTAGAC1255.180482E-946.399912
TACTACT700.001055750641.4284934
TAGACGT4700.038.5637554
TCTAGGG1209.547011E-636.249932
TCTAGAC2850.035.6139682
CGATCTT22450.035.200385
GGCTAGG1251.2604656E-534.809591
ACTGATA1051.8513908E-434.5237468
ATCTAGG1501.1287866E-633.842661
TAGAGTG1501.1312386E-633.8332675
CTAGAAA1301.6518794E-533.4614754
CCGATCT79450.032.7595334
CTTCCGA80500.032.611471
TTCCGAT80750.032.2321362
TAATCCG1352.1384534E-532.222165
TCCGATC81450.032.0441363
GCTAGGG1851.6386912E-731.351292
TCTAGAA1652.380877E-630.7575153
GATCTTT14800.029.8816996
Produced by FastQC (version 0.11.2)