FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6g11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6g11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816016
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA59750.2121791921636809No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC51020.18117794785256902No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC46290.16438116828881655No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA45620.16200192044363385No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT40480.143749183243277No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT37700.13387708024386225No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT37680.13380605792012545No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT34800.1235788433020267No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT33500.1189623922591349No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA32980.11711581184197817No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA31110.11047522457258765No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC30930.10983602365895648No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT30160.10710166419508979No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT29560.10497099448298589No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC28660.10177498991483004No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA28640.10170396759109322No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA28320.10056761041130448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA90200.048.7922481
TTCCGAT93200.047.2961122
CCGATCT93750.047.0186424
GGGCGAC16950.041.4896557
CGATCTG41100.040.571765
TCCGATC134350.033.0796243
AGACGCG11950.032.761494145
CGATCTA23000.028.0543355
CGATCTT33600.027.4032615
GGCGACC25500.027.2941048
GCGACCC26050.027.2744589
CGATCTC46050.027.2366855
CCTATAA38850.026.3145981
TAAGGTG25350.025.4536385
CTATAAG40200.025.2487432
TCGCGCG18650.024.8793439
AGGTGCC24900.023.875497
TATAAGC43600.023.2798043
TAGGACA13850.023.032484
ATAAGGT27600.022.853254
Produced by FastQC (version 0.11.2)