FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699695
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA77770.2880695782301334No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC69590.25776985918779716No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC66540.24647228668423657No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA63960.236915651582864No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT57260.21209803329635385No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT52410.19413304095462636No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT50430.18679887913264276No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA45710.16931542266811622No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA44880.1662410012982948No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT43430.16087002420643814No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG42170.1562028303197213No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA42060.15579537688516665No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT40900.15149859521168133No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT39530.14642394789040986No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA39300.1455719997999774No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT38300.14186787766766246No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC38180.14142338301178467No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT37370.13842304408460954No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC36060.13357064409127697No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA35570.13175562424644266No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC35200.1303850990574861No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG33950.12575494639209245No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG33800.1251993280722452No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT33780.1251252456295989No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG33460.12393992654725812No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA32650.120939587620083No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA31840.11793924869290791No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG31670.11730954793041436No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA30000.11112366396944841No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG29650.10982722122313818No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCA29270.1084196548128585No Hit
GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC27720.10267826550777032No Hit
CAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGC27350.10130774031881379No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT27180.10067803955632025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT71800.054.2234232
CTTCCGA72450.053.243511
CCGATCT75100.051.7442324
CGATCTG34350.047.7000475
CGATCTA17550.034.7007835
ACACATA12350.032.874424145
GTATATA6100.032.094261
GGGCGAC21100.031.2677087
CGATCTC32150.030.8941845
TAGAGTG23150.030.3779075
CTTATAA11950.029.727972145
TCCGATC131750.029.715313
CCATACT28500.027.7280142
ACCATAC28900.027.3477781
AAATTAG23500.027.1524011
TCTTCCG45900.026.8517995
TTAGAGT26300.026.739494
CATACTC30750.026.6422233
TCCCCCC29100.026.4088828
CGATCTT25800.025.8526615
Produced by FastQC (version 0.11.2)