FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2277047
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA48150.21145808584539538No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC40570.17816935706641102No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC39800.17478778435403397No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA37060.16275465548141957No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT33560.14738387042516032No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT31920.14018155971308452No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT29840.13104692173679333No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA28520.12524993994414696No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA27860.12235144904782379No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT26290.11545655403687319No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA25740.11304114495660389No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA25530.11211889785322832No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT25490.11194323173829965No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT23500.10320384252059794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA60900.051.6723021
CCGATCT65450.048.739464
CGATCTG31100.047.5562325
TTCCGAT66750.047.1385422
GGGCGAC10500.033.142837
TCCGATC98600.032.4999733
TAGAGTG17150.030.8600355
CGGGCCG4650.028.064495145
AGGTGCC19100.026.9502417
CCTATAA31150.026.5357971
CTAGGGG4100.026.524373
ATAAGGT22850.025.7001974
TTAGAGT21750.025.6666494
CTATAAG34000.024.9485112
CGATCTA18150.024.7658215
TAAGGTG21900.024.49775
GCGACCC15200.024.325649
GTGCTCA20950.023.8782639
AAATTAG21350.023.0938511
CATAAGG24250.023.0206013
Produced by FastQC (version 0.11.2)