FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776317
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA18970.2443589409996174No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15490.19953189225535448No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA13790.17763362131706506No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC13720.1767319278078414No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT12630.16269127173564407No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT12230.15753873739722304No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11050.142338761098881No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA10420.13422351951586786No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT9530.12275913061288107No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG9480.12211506382057845No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9380.1208269302359732No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT9200.11850828978368373No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA8770.11296931536988113No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8280.10665746080531534No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA8230.10601339401301271No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC7930.10214899325919696No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC7770.10008797952382854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA20950.056.4081151
CCGATCT22250.052.786524
TTCCGAT22400.052.7566952
CGATCTG9800.048.826535
TCGCGCG3150.036.8253979
GTCGCGC3400.034.1176458
ACGGACG2850.033.070175145
GGGCGAC3950.033.0379757
TCCGATC35950.032.8720443
GGTCGCG3850.032.012997
AGGTCGC3850.032.012996
GCGCCAT3050.030.901646
CGATCTC10800.029.5370375
CGATCTA5700.029.2543855
ACCATAC6500.029.01
TAGGCGG1000.00607363129.04
TATAGGT4150.027.9518073
ATAACGC3153.45608E-1127.6190479
GTATATA2654.5547495E-927.3584921
ACACATA4800.027.1875145
Produced by FastQC (version 0.11.2)