FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601952
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA32440.2025029464053854No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC26950.1682322566468908No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC26780.1671710513173928No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA25090.15662142186532432No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT24030.15000449451668965No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT22710.14176454725235213No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA19230.1200410499190987No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT19170.11966650686162882No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT17880.11161383112602624No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG17510.10930414893829529No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA17020.10624538063562453No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT16920.10562114220650806No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA16880.10537144683486148No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA16290.10168844010307425No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC16160.10087693014522282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA41300.055.1312641
CCGATCT42250.053.5400054
TTCCGAT45400.050.1446152
CGATCTG20800.047.4052125
GGGCGAC9050.033.6463287
TCCGATC68750.033.219143
CAGGGGC4050.028.6428014
TAGAGTG11600.026.2507575
CGATCTA11450.025.9614495
TACTATA4500.025.778522
AGGTGCC16200.025.061677
CGATCTC22300.024.7092345
TCCCCCC12650.024.644218
CGATCTT18150.024.3670925
GGCGACC12200.024.3646978
CCAGGGG5400.024.1673643
GCGACCC13200.023.6173699
TATATAC5850.023.5476883
ATACTCC14200.023.4865914
TTAGAGT14050.023.221314
Produced by FastQC (version 0.11.2)