FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477389
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA35830.24252245007915993No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC29550.2000150265096058No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC29280.1981874780440358No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT27410.18553001274545838No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA27300.1847854559631891No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT23710.16048583006912873No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA22150.14992666115694647No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT21970.14870829551323314No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT18860.12765764466907498No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA18150.12285186907442794No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG18000.12183656437133347No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA16860.11412024862781568No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT16690.11296956996430865No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA16340.11060052565708828No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA16080.10884066417172458No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT15910.10768998550821753No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC15490.10484713233955309No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG15140.10247808803233271No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT15130.10241040105212643No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA15030.10173353125006346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA37850.048.4691731
TTCCGAT38750.047.8967172
CCGATCT39500.046.253114
CGATCTG19350.045.7105455
GGGCGAC10250.035.3658147
TCCGATC63750.029.3411433
TCGCGCG6500.027.8845849
GGCGACC12600.027.6190178
TAGAGTG12850.027.081685
GCGACCC12850.026.5174799
TAAGGTG16100.026.1179835
AGGTGCC15750.025.3174327
CCTATAA23200.025.00421
ATAATAC4450.024.4381733
CGATCTA9900.024.166645
TTAGAGT14200.023.996454
CTATAAG25150.023.9264132
GTCGCGC7300.023.835598
AAATTAG13400.023.809971
TCTTCCG26800.023.8059435
Produced by FastQC (version 0.11.2)