FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178422
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7490.41979128134422883No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.18999899115579919No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3040.17038257613971372No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2720.152447568125007No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2490.13955678111443656No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.1384358431135174No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA2380.13339162210938113No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2150.12050083509881068No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.11601708309513402No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCAA604.934091E-448.3333329
ATAATAC700.001054306341.428573
TGTAGAC1053.8064281E-641.428572
ATCTAGC1105.239999E-639.5454527
CTAGTAG951.0254984E-438.157893
GTTTAAG1354.940903E-737.5925941
TTTAAGC1707.761446E-834.117652
CATATCA1501.1269749E-633.8333368
AGCATAT1501.1269749E-633.8333366
GCATATC1501.1269749E-633.8333367
TAAGCAT1751.0012991E-733.1428574
GGAAGGA1102.4281746E-432.9545444
CGATCTG12950.032.4710435
CTAGACG1352.1324126E-532.222223
TAGACGT2705.456968E-1232.222224
TCTAGAC1801.2823148E-732.222222
ATCTTGC900.003620307432.222227
CCGATCT32500.031.4538464
AAGGTGC1402.7341504E-531.0714286
TATAAGC2101.4259058E-831.0714283
Produced by FastQC (version 0.11.2)