FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6f11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6f11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733106
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA13970.19055907331272692No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10980.149773702575071No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10430.1422713768540975No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9860.13449623928872495No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT9070.12372017143496303No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8850.12071924114657362No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8080.11021598513721073No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA7770.10598740154902565No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT7440.10148600611644155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC4350.048.3333367
TAGGGCC657.3352974E-444.6153834
TTCCGAT30900.040.121362
CTTCCGA31500.039.58731
CCGATCT32200.038.2763944
CGATCTA6300.033.3730165
GCGACCC5900.033.1779679
TAATAGT1102.4355177E-432.9545484
CGATCTG15700.032.324845
GGCGACC6050.031.1570248
TCCGATC41100.030.3406333
CAGGGGC2802.6193447E-1028.4821434
TACTATA1606.825691E-527.18757
CCTATAA8750.026.5142861
TTAGAGT5500.026.3636364
CGGGCCG1658.415564E-526.363636145
TAGAGTG5100.025.5882365
TATAAGC9800.025.153063
CTATAAG9250.025.0810812
TACACGG1500.001493893624.1666665
Produced by FastQC (version 0.11.2)